30.009_T067_-ANA-screening-_Quantitative-14-antibodies__NTest_Sep_2023.webp
ANA screening (Quantitative 14 antibodies)
In normal conditions, our body has no immunity to its own tissues. But for whatever reason, the immune system's control mechanism is abnormal. There is a response by creating Antibodies for the tissue or components of their own. Such a condition is called Autoimmunity. This group of people will be detected as Anti Nuclear Antibodies (ANA).
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         In normal conditions, our body has no immunity to its own tissues. But for whatever reason, the immune system's control mechanism is abnormal. There is a response by creating Antibodies for the tissue or components of their own. Such a condition is called Autoimmunity. This group of people will be detected as Anti Nuclear Antibodies (ANA).  

        The ANA test is used to diagnose ANA-associated rheumatic diseases such as Systemic lupus erythematosus (SLE), Systemic sclerosis (scleroderma), Mixed connective tissue disease (Connective tissue disease), Primary Sjögren's syndrome (Sjögren's syndrome), Polymyositis/dermatomyositis (muscle inflammation), etc. The symptoms of this group of diseases can range from joint and muscle pain, fever, body rash, fatigue, dry eyes, oral inflammation, hair loss, and chest pain. Recommend seeing a doctor to diagnose the cause of the disease

        ANA screening with IFA (Indirect immunofluorescence assay) is considered the gold standard method for ANA screening. The IFA test is highly sensitive (81.7-98.7%), but at the same time may lack specificity (61.0-88.6%) for autoimmune rheumatic disease.

At present, there are other methods of ANA screening, such as Fluoro-enzyme Immunoassay (FEIA), which have the following advantages:
• The results of 14 Antibodies can be reported as Quantitative Results with a fully automated system.
• Used to screen patients for CTDs with a single test
• High Specificity (Specificity 84% สำหรับการตรวจโรคกลุ่ม CTDs) ช่วยในการลด False positive
• High Specificity (84% for CTDs) helps reduce false positives.
• High Sensitivity (92% for CTDs) can detect specific autoantibody in asymptomatic patients.
• Can be used for both Diagnosis, Prognosis, and Follow up results.
image.webpN Health offers ANA screening (Quantitative 14 antibodies)(CTD screening) with the following detailsimage.webpที่มา :
- https://www.lupus.org/resources/the-antinuclear-antibody-ana-test
- https://www.cdc.gov/lupus/basics/symptoms.htm
- https://ard.bmj.com/content/early/2018/12/08/annrheumdis-2018-214805
- Van der Pol P (2017) Analytical and clinical comparison of two fully automated immunoassay systems for the detection of autoantibodies to extractable nuclear antigens. Clin Chim Acta. 2018 Jan;476: 154-159.doi: 10.1016/j.cca.2017.11.014. Epub 2017 Nov21